Composite

Part:BBa_K1758354

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-09-08)

Nickel responsive promoter with T7-promoter and UTR-sfGFP

nickel induceble promoter under the control of a T7 promoter wit a 5´untranslated region and sfGFP for detection.


Usage and Biology

This part is essential for our in vitro characterization of our nickel sensor. The edition of T7 promoter to BBa_K1758353 enables us to characterize tihs sensor in our CFPS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 198


[edit]
Categories
//function/sensor/metal
Parameters
None